CD11b for BMDC

February 14, 2018

Immunology
The Jackson Laboratory for Genomic Medicine
Lab Manager, RA III

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

PE/Cy7 anti-mouse/human CD11b Antibody

Catalog Number:

101215

This antibody was used to stain BMDC for CD11b and other surface molecules to make sure they differentiated correctly in culture during a survival assay.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse BMDC

Primary Incubation

1/50 dilution in PBS+ 2%FBS for 15min at RT at dark.

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII

Results Summary

Good staining to separate 2 populations of BMDC CD11b high and intermediate

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clear separation, bright

The Bad

None

The Bottom Line

Good antibody to stain CD11b positive dendritic cells

Share your experience with other scientists. Write a Review! »

Join the discussion