Effective CD3 Antibody For FACS

January 09, 2018

BlueskyReddit
Pediatrics
Medical College of Wisconsin
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC anti-human CD3

Catalog Number:

344804

This antibody has been used to detect human CD3 by FACS on Jurkat cells and primary T cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

T cells, Jurkat cell line

Primary Incubation

1:100, 30 minute incubation at 4 degrees

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

This antibody gives a distinct peak of CD3+ cells, which can be easily distinguished from CD3- cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very effective for flow cytometry

The Bad

N/A

The Bottom Line

This antibody works well for me

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