Brightly Staining IL-5 Antibody

January 24, 2018

BlueskyReddit
Cancer Immunology and Virology
Dana-Farber Cancer Institute
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse/human IL-5 Antibody

Catalog Number:

504311

This antibody was used to identify CD4+IL-5+ cells in the mouse lung in an allergen exposure model.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single cell suspension of mouse lung cells

Primary Incubation

1:200, 30 mins, 4 C

Blocking Agent

Mouse FcX

Secondary Incubation

None

Tertiary Incubation

None

Detection

Violet laser (BD Fortessa)

Results Summary

Specific detection of IL-5+ cells was achieved using this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright stain and specific detection

The Bad

None

The Bottom Line

Ideal for detecting IL-5+ cells in mouse cells.

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