Good Antibody For Staining CD24+ Cells

February 09, 2018

Cancer Immunology and Virology
Dana-Farber Cancer Institute
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BUV737 Rat Anti-Mouse CD24 Clone M1/69

Catalog Number:

565308

This antibody was used to identify CD24+ cells among CD11c+ cells in mouse lungs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single cell suspension of mouse lung cells

Primary Incubation

1:300, 25 mins, 4C

Blocking Agent

Mouse FcX

Secondary Incubation

None

Tertiary Incubation

None

Detection

UV laser (BD Fortessa)

Results Summary

Nearly all of the CD11c+ cells in mouse lungs were found to express CD24.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use antibody, and gives a good separation of positive and negative populations

The Bad

None

The Bottom Line

Good antibody to be used in a multi-color flow cytometry panel.

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