Great Alternative for PE/Cy7

February 08, 2018

Cancer Immunology and Virology
Dana-Farber Cancer Institute
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Anti-TCRγ/δ-PE-Vio770, mouse (clone: GL3)

Catalog Number:

130-104-042

The goal of this experiment was to analyze the frequency of gdT cells among CD3+CD4-CD8- T cells in the lung.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single cell suspension of mouse lung cells

Primary Incubation

1:250, 4 C, 30 mins

Blocking Agent

Mouse FcX

Secondary Incubation

None

Tertiary Incubation

None

Detection

Yellow-Green laser (BD Fortessa)

Results Summary

Among CD4-CD8- T cells in the mouse lung, 20-30% of these cells were found to be positive for TCR g/d.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Stains well and easy to use.

The Bad

None

The Bottom Line

Good alternative for the PE/Cy7 channel.

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