Excellent Flow Cytometry Antibody For Mouse CD4

February 09, 2018

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Invitrogen Antibodies

Product Name:

CD4 Monoclonal Antibody (GK1.5), PE-Cyanine7, eBioscience™

Catalog Number:

25-0041-81

I used this antibody to identify Tregs in spleen, thymus and peripheral blood based on CD4+CD25+Foxp3+ expression pattern. This antibody stained well and gave a nice distinct population

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse T-cells

Primary Incubation

1:400 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

This antibody stained well and gave a clear distinct population. I was able to generate presentable figures without much optimization hurdles.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Ease of optimization and reproducible results

The Bad

Nothing

The Bottom Line

Good quality antibody and worth buying it

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