Antibody For Mouse CD25 Staining

January 16, 2018

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Biolegend

Product Name:

APC anti-mouse CD25 Antibody

Catalog Number:

102011

I used this antibody to characterize mouse Tregs based on CD25 and Foxp3 expression. I was looking for a cheaper antibody and I found this one from biolegend. This antibody stained well and gave us nice distinct population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Thymocytes

Primary Incubation

1:400 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

This antibody stained well and gave a clear distinct population. I was able to generate presentable figures without much optimization hurdles.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Cheap and reproducible results

The Bad

Nothing

The Bottom Line

Good quality antibody at cheaper price

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