Excellent Antibody For Human OX40 Staining

January 05, 2018

BlueskyReddit
Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Biolegend

Product Name:

PE anti-human CD134 (OX40) Antibody

Catalog Number:

350003

I used this antibody to characterize OX40 expression in human thymus. especially thymic Tregs. This antibody stained well and gave us reproducible results.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human thymocytes

Primary Incubation

1:200 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

This antibody stained well and gave a clear distinct population. I was able to generate presentable figures without much optimization hurdles.

DOI or PMID #

NA

Additional Notes

Surface staining of CD134 before Fixation gives better results

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Nothing

The Bottom Line

Good quality antibody and worth buying it

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