Bioruptor For Protein Extraction

I'm interesting in understanding the molecular mechanism of hedgehog signaling pathway. In this experiment, my goal is to identify binding partners of a cilia localized protein in cultured cells by Mass Spectrometry. The first step in this process involves extraction of proteins from ~5 mg cell pellet. Efficient protein extraction and removal of DNA is critical for this initial step. Diagenode's Bioruptor was used for this process.