Exceptionally Bright IL-10 Antibody

Autoimmune Genetics
KU Leuven
Senior Post-doctoral Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Dazzle™ 594 anti-mouse IL-10 Antibody

Catalog Number:

505034

Our goal was to establish conditions for highly reliable cytokine staining in a high throughput setting. I tested the same clone of anti-IL-10 conjugated to several of the brightest fluorophores currently available. This one provides a signal that rivals PE, with minimal non-specific binding and spreading of the negative population. Furthermore, this conjugate provided similar staining intensity even when the antibody concentration varied by over an order of magnitude (between 1:100 and 1:2000). This makes it highly resistant to minor changes in researcher handling.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymphocytes

Primary Incubation

16h, 4C, 1:1000

Blocking Agent

2.4G2 hybridoma supernatant

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS Symphony

Results Summary

Bright, specific staining

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright, specific, can be highly diluted

The Bad

Can only be used on a machine equipped to detect PE-CF594/PE-Dazzle594/PE-TexasRed.

The Bottom Line

Excellent product. Probably the best IL-10 antibody on the market.

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