Anti-Mouse MHC II (I-A/I-E) Antibody

Medicine
Northwestern University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 700 anti-mouse I-A/I-E Antibody

Catalog Number:

107622

In this experiment we were examining antigen presenting cells in the lung in WT and KO mice.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Digested lung from mouse

Primary Incubation

0.6 ul per 100 ul reaction, incubated 30 minutes at 4 degrees in the dark

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II

Results Summary

This antibody works well to detect MHC II on cells isolated from mice.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It works and the signal is strong

The Bad

None

The Bottom Line

This antibody is useful for detecting MHC II by flow cytometry.

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