Anti-Mouse IL-5Ra (CD125) Antibody

January 31, 2018

BlueskyReddit
Medicine
Northwestern University
Graduate Student

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Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Alexa Fluor® 488 Rat Anti-Mouse CD125

Catalog Number:

558533

In this experiment we were examining stem cells in the bone marrow of WT and KO mice.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Bone marrow

Primary Incubation

0.5 ul per 100 ul reaction, incubated 30 minutes at 4 degrees in the dark

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II

Results Summary

This antibody works well for detecting IL-5Ra.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It works and the signal is strong

The Bad

None

The Bottom Line

This antibody is useful for detecting IL-5Ra.

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