Invitrogen Antibodies
CD34 Monoclonal Antibody (RAM34), eFluor 450
48-0341-82
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Browse Similar Products: CD34 Flow Cytometry Antibodies
In this experiment, we were comparing stem cell populations in bone marrow of WT and KO mice.
Flow Cytometry
Bone marrow from mouse
2.5 ul per 100 ul reaction, incubated 30 minutes at 4 degrees in the dark
FC Block
None
BD LSR II
The signal is weak, but there is a positive signal. My data looks similar/better than the data shown on the eBioscience product page. I would suggest using a brighter fluorophore like PE or APC.
N/A
The antibody works.
The positive signal is very weak.
I would suggest using a brighter fluorophore for staining the bone marrow.