Invitrogen Antibodies
Granzyme B Monoclonal Antibody
25-8898-82
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Browse Similar Products: Granzyme B Flow Cytometry Antibodies
My research aim is to investigate the response of the immune system after immune-therapy. This antibody is a good GranzymeB marker, that has been tested on lymphocytes extracted from spleen, lymph nodes and glioma tumor-bearing mice with the aim to evaluate activation in CD4 and CD8 T cells.
Flow Cytometry
Mouse Splenocytes, TILs
30min
Fc
N/A
Fortessa
After cell extraction and blocking with Fc Block, wash your sample 2x with washing buffer, stain for 30min RT with your extracellular staining, wash 2x and then proceed with intracellular staining protocol for GranzymeB (1:200).TILs extract from brain-tumor mice and spleenocytes were gated for Live/CD45+/CD3+/CD8+ and Live/CD45+/CD3+/CD4+
High positive population
Price
PE-Cy7 anti-mouse GranzymeB has been chosen because is a bright antibody with a good MFI and fit in my multicolor panel designed for T cell analysis.