Invitrogen by Thermo Fisher Scientific
ERCC ExFold RNA Spike-In Mixes
4456739
Usually the quantitative expression of the genes requires a standard reference gene to minimize the cellular, RNA quality and handling error. Generally, when handling serum sample RNA, we really don't have a standard deference RNA to normalize the data. ERCC ExFold RNA Spike-In Mix provides us with external RNA which can be added to the serum samples before isolating total RNA. This external RNA acts as the reference genes for analysis of gene expression using NGS.
NGS / Real-time PCR data analysis
Human serum/plasma
During isolation of the RNA from the serum/plasma samples, a known concentration of the external RNA is added to the serum/plasma samples and total RNA is isolated from serum samples. Then we proceed for preparation of the RNA libraries for the samples.
You have to use this kit only you are working with serum/plasma/milk samples.
After preparation of the RNA libraries from the external RNA added total RNA, the libraries went on for RNA sequencing. Once the sequencing run is completed, the results must be mapped and counted using ERCC reference file.
N/A
A reliable method for RNA quantification.
Costly
If you are looking for a reference control for serum samples, you can consider this kit.