Biolegend
TCR va7.2 BV605
351720
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To test if Biolegend's anti-human va7.2 TCR BV605 works, I stained human PBMC with this antibody and did a serial titration. This trial experiment suggests that this antibody can be used to identify potential Mait cells in human PBMCs.
Flow Cytometry
2e6 Human PBMC in FACS buffer
30min at RT in the dark
NA
BD Fortessa 4
Cells positive for TCR va7.2 were identified.
I titrated the antibody by performing seven 2-fold dilution starting at 1:20.
Antibodies work
I tested this antibody on PBMC and T cells positive for TCR va7.2 were identified.