Anti-Mouse/Human CD45R/B220 Antibody

November 07, 2017

Harvard Medical School
Harvard
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC anti-mouse/human CD45R/B220 Antibody

Catalog Number:

103212

I am studying molecular anomalies in signaling pathways leading to cancers and development of anti-cancer immune therapies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine lymphocytes

Primary Incubation

1:100 dilution for 1 hour on ice

Blocking Agent

Blocking with 2% BSA and Fc block (1:100)

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS

Results Summary

Efficient B220 expression detection on mouse immune cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good antibody for efficient detection of B220 on mice immune cells.

The Bad

None

The Bottom Line

Expressed on B cells, T cell subset, NK cell subset.

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