CD3 Antibody

Microbiology & Immunology
University of Rochester Medical Center
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC conjugated anti-mouse CD3 antibody

Catalog Number:

100203

CD3 is expressed in a variety of lymphocyte species, so CD3 antibody in flow cytometry analysis is frequently used to specifically identify lymphocyte subsets together with antibodies to other lymphocyte markers such as CD4 or CD8. FITC-conjugated anti-mouse CD3 antibody from Biolegend showed good performance in marking lymphocyte population in splenocytes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

100 ng antibody in 100 ul of cell suspension in PBS containing 10% FBS, 30 min at 4 degree

Blocking Agent

10% FBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry using FACS Calibur

Results Summary

Lymphocytes in spleen were efficiently stained with the FITC-conjugated anti-mouse CD3 antibody and analysed using flow cytometry.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Good performance and an affordable price

The Bad

None

The Bottom Line

Useful to specifically detect lymphocytes in flow cytometry analysis

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