Good Actin Antibody

Pharmaceutical Technology
CIBER BBN
Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-beta Actin antibody

Catalog Number:

ab16039

We use this actin antibody as housekeeping in our western blotting assays. This antibody works very well.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Different human and mouse cell lines

Primary Incubation

1:5000

Blocking Agent

Non fat milk

Secondary Incubation

1:10000

Tertiary Incubation

None

Detection

ECL western blooting substrate

Results Summary

The antibody stains actin at 42kDa size in all the samples and it can be used as housekeeping in the western blot analysis of all type of eukaryotic cell lines.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very sensitive

The Bad

None

The Bottom Line

It works very well

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