Invitrogen Antibodies
FOXP3 Monoclonal Antibody (PCH101), FITC
11-4776-42
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I used to this antibody to characterize human Tregs based on CD4+CD25+FOXP3+ definition. This antibody worked well and gave a nice distinct population.
Flow Cytometry
Human thymocytes
1:100 for 3h at 4 degree
0.5% BSA
NA
Flow cytometry
This antibody stained well and gave a clear distinct population. I was able to generate presentable figures without much optimization hurdles.
Ease of optimization and reproducible results
Expensive
Good quality antibody and worth buying it