Excellent Flow Cytometry Antibody For Human CD45

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Invitrogen Antibodies

Product Name:

CD45 Monoclonal Antibody (HI30), PE-Cyanine7

Catalog Number:

MHCD4512

I used this antibody to characterize thymic epithelial cells where I used CD45 to eliminate hematopoietic cells. We defined thymic epithelial cells as CD45-EpCAM+ cells. This antibody stained well and gave a nice distinct population

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human thymocytes

Primary Incubation

1:400 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

This antibody stained well and gave a clear distinct population. I was able to generate presentable figures without much optimization hurdles.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Ease of optimization and cheap

The Bad

Nothing

The Bottom Line

Good quality antibody and worth buying it

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