ADO Antibody Works in Western Blot

Pediatrics
University of Colorado Denver
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Boster Biological Technology

Product Name:

Anti-ADO Picoband™ Antibody

Catalog Number:

PB10032

We are studying the metabolism of amino acid and we are interested in knowing if the pathway involved this protein has changed under the background of a knockout mouse. The western blot image for this antibody looks clean and the signal looks strong. Equally important, the price is generally lower than other companies.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human liver, rat liver, mouse liver, mouse kinday, and mouse brain

Primary Incubation

At 4 degrees celcius overnight

Blocking Agent

5% milk

Secondary Incubation

At room temperature for 1 hour

Tertiary Incubation

None

Detection

Typhoon 940

Results Summary

This antibody works ok in western blot experiments with human, rat, and mouse tissues.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The band shown on western blot is at the right size and the intensity is strong enough.

The Bad

Extra non-specific bands

The Bottom Line

It looks like it recognizes the right protein and can be used in experiment.

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