Excellent Flow Cytometry Antibody For Mouse/Human Ki67

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Biolegend

Product Name:

Alexa Fluor® 488 anti-mouse/human Ki-67 Antibody

Catalog Number:

151204

I used this antibody to analyze proliferation marker Ki67 expression in mouse T-cells. This antibody stained well and gave a clear distinct population. I was able to generate presentable figures without much optimization hurdles.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Thymocytes

Primary Incubation

1:200 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

This antibody stained well and gave a clear distinct population. I was able to generate presentable figures without much optimization hurdles.

DOI or PMID #

NA

Additional Notes

Using isotype control will help in normalization

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Smaller pack size is not available.

The Bottom Line

Good quality antibody and worth buying it

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