Biolegend
PE anti-mouse CD120b (TNF R Type II/p75) Antibody
113405
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I used this antibody to characterize TNFR-II expression in mouse Tconv and Treg cells. This antibody worked well and gave nice staining.
Flow Cytometry
Mouse splenocytes
1:200 for 3h at 4 degree
0.5% BSA
NA
Flow cytometry
This antibody stained well and gave a clear distinct population. I was able to generate presentable figures without much optimization hurdles.
This picture was generated using Foxp3.GFP cells. Cells were not fix permeabilized.
Ease of optimization and cheap
Nothing
Good quality antibody and worth buying it