Excellent Flow Cytometry Antibody For Mouse GITR

October 24, 2017

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Invitrogen Antibodies

Product Name:

CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), PE

Catalog Number:

12-5874-82

I used this antibody to characterize GITR expression in mouse TConv and Treg cells. This antibody worked well and gave a nice distinct population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:200 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

This antibody stained well and gave a clear distinct population. I was able to generate presentable figures without much optimization hurdles.

DOI or PMID #

NA

Additional Notes

This picture was generated using Foxp3-GFP cells. Cells were not fix permeabilized.

Related Categories

Image Gallery

Summary

The Good

Ease of optimization and reproducible results

The Bad

Expensive

The Bottom Line

Good quality antibody and worth buying it

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