Invitrogen Antibodies
CD25 Monoclonal Antibody (BC96), eFluor 450
48-0259-42
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Browse Similar Products: CD25 Flow Cytometry Antibodies
I used to this antibody to characterize human Tregs based on CD4+CD25+FOXP3+ definition. This antibody worked well and gave a nice distinct population.
Flow Cytometry
Human thymocytes
1:200 for 3h at 4 degree
0.5% BSA
NA
Flow cytometry
This antibody stained well and gave a clear distinct population. I was able to generate presentable figures without much optimization hurdles.
Addition of CD25 stain before fixation gives better results
Ease of optimization and reproducible results
Expensive
Good quality antibody and worth buying it