BioLegend
anti-CD27 mouse/human BV510 antibody
124229
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We are an immunology lab studying CD8 T cell memory differentiation. CD27 is a hallmark memory marker. We performed adoptive transfer in vivo experiments, and harvest spleens during the acute response of infection. I used this antibody in my T cell panel to identify memory precursors. I chose this product since it fit well in my 12 color panel. Biolegend was one of the few vendors offering this marker with this particular clone and color.
Flow Cytometry
Murine Splenocytes
1:500. 30 mins, 4 deg incubation
FC blocking in FACS buffer (2% FBS, .2% NaAzide/PBS)
None
Flow cytometer
I analyzed the adoptively transferred cells during the acute response. Further analysis of the cells led to the identification of CD27+CD8+KLRG1- memory precursors.
N/A
The signal was very strong and was able to identify population using a nice dilution.
Great product! Highly recommend it!