The Good
Very specific, intense signal, very little is used for each blot since it can be diluted 1:5000
The Bad
The signal is sometimes so intense that is can make it difficult to detect other proteins if you are probing for multiple proteins on the same membrane.
The Bottom Line
This secondary antibody is very reliable, specific, and sensitive to ECL substrates. It is very economical and effective at consistently detecting proteins that are probed with rabbit primary antibodies.