Halt Protease Inhibitors

University of South Alabama
Cancer Biology
Graduate Student

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Company:

ThermoFisher Scientific

Product Name:

Halt Protease Inhibitor Cocktail

Catalog Number:

78430

The Halt Protease Inhibitor Cocktail is an extremely valuable reagent for a variety of applications such as mammalian cell signaling studies, recombinant protein purification, and others. It contains a mixture of 6 different protease inhibitors to prevent proteolysis of proteins in your samples. It can be ordered in a 1 mL, 5 mL, or 10 mL container, along with single use vials if desired. This gives the user the ability to add the inhibitor cocktail fresh to your lysis buffer immediately prior to performing your experiment. I have used this

Experimental Design and Results Summary

Application

Cell Lysate Collection

Starting Material

Mammalian cell lines

Protocol Overview

This reagent is sold as a 100X solution that is added freshly to your lysis buffer (10 uL per 1 mL of buffer). The lysis buffer can then be used with the peace of mind that proteins in your sample will not be degraded by proteases.

Tips

Add the cocktail fresh to your buffer. Do not freeze aliquots of it.

Results Summary

The protease inhibitor cocktail effectively prevents proteolysis of proteins in your samples, which can be verified by western blot.

DOI or PMID #

N/A

Additional Notes

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Summary

The Good

Easy to use, very effective, stable at 4 degrees Celsius.

The Bad

It is expensive even in small quantities.

The Bottom Line

This protease inhibitor cocktail is extremely effective and easy to use. I have had success with it while collecting lysates from mammalian cell lines, along with purifying recombinant GST fusion proteins from bacterial lysates.

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