Rat Skin Wound CD68 Staining

October 05, 2017

UIC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

Mouse anti Rat CD68 clone ED1

Catalog Number:

MCA341GA

The aim is to understand the role of macrophages in wound healing. The goal is to identify infiltrated macrophages in the skin tissue. The antibody seems to work well to stain macrophages in rat skin wounds.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Rat skin wound

Primary Incubation

Overnight 4C

Blocking Agent

Goat serum 10%

Secondary Incubation

45min RT

Tertiary Incubation

None

Detection

Goat anti-mouse Alexa Fluor® 594

Results Summary

Specific staining with less background

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong and specific staining

The Bad

None

The Bottom Line

No complaints

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