Anti-Mouse CD62L (PE-Cy7) Antibody

October 02, 2017

BlueskyReddit
Cell Biology
La Jolla Institute for Allergy and Immunology
Instructor

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE/Cy7 anti-mouse CD62L Antibody

Catalog Number:

104418

The aim was to study T helper cell subsets. The specific goal was to examine the status of T cell activation. I selected this product because it was of high quality and is cost effective.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocyte

Primary Incubation

1:100 (0.4ul of antibody in 40ul of sample)

Blocking Agent

5% rat serum

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

PE-Cy7

Results Summary

CD62L expression from mouse CD4 T cells was determined by flow cytometry.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

High quality with low cost

The Bad

None

The Bottom Line

This antibody is working well in flow cytometry

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