CD11c PerCP-Cy5.5 Antibody

October 02, 2017

Immunology
University of Washington
Senior Research Fellow

Overall

Quality of Results

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Company:

eBioscience™ from Invitrogen Antibodies

Product Name:

anti-mouse CD11c PerCP-Cy5.5

Catalog Number:

45-0114-80

I study host-pathogen interactions. In this experiment, I was phenotyping splenocytes from mice after virus infection. I selected this antibody to help distinguish populations of immune cells. This antibody was selected because I have previously used antibodies from eBioscience that have worked well. eBioscience also offers a wide variety of antibodies and now they are available through the Thermo Fisher website.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Splenocytes

Primary Incubation

1 uL per roughly 1e6 cells in 100 uL total volume

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSRII

Results Summary

This antibody showed a population of CD11c+ cells from the spleen.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works on mouse spleen cells

The Bad

Not as clear of a separation as might be desired

The Bottom Line

Moderately priced antibody that works for basic phenotyping

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