Biolegend
PE/Cy7 anti-mouse IL-17A Antibody
506922
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The aim was to study Th1/Th17 development. The specific goal was to examine Th1 cells by IFN-gamma expression. I selected this product because it was of high quality and is cost effective.
Flow Cytometry
Lamina propria of large intestine from mouse
1:100 (0.4ul of antibody in 40ul of sample)
1X Permeabilization buffer (BD Bioscience or ThermoFisher)
NA
PE-Cy7
This antibody is good enough to detect Th17 cells from mouse CD4 T cells
Quality (high sensitivity)
Before intracellular staining, cells were activated by PMA, ionomycin, and GolgiStop for 3 hrs.