Foxp3-FITC Antibody

Cell Biology
La Jolla Institute for Allergy and Immunology
Instructor

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies

Product Name:

FOXP3 Monoclonal Antibody (FJK-16s), FITC, eBioscience™

Catalog Number:

11-5773-82

The aim was to study the development/function of regulatory T cells (Treg). The goal was to distinguish the Treg population by Foxp3 expression. The reason for selecting this product is because the quality is the best. This clone is best among other monoclonal antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 (0.4ul of antibody in 40ul of sample)

Blocking Agent

1X Permeabilization buffer (ThermoFisher)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FITC

Results Summary

Foxp3 T cells were detected by this antibody, but the intensity of FITC is somewhat dimmer than other APC or PE-conjugated antibodies.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Specificity

The Bad

Fixation/Permeabilization is required

The Bottom Line

For proper staining, Foxp3 staining buffer set should be needed.

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