Ki-67 Antibody

Cell Biology
La Jolla Institute for Allergy and Immunology
Instructor

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Alexa Fluor® 700 Mouse anti-Ki-67 Clone B56

Catalog Number:

561277

The aim was to study the development/function of regulatory T cells (Treg). The specific goal was to examine the proliferative capacity of Treg cells by Ki-67 expression. The reason for selecting this product was because it was recommended by colleagues.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse CD4 T cells

Primary Incubation

1:20 (2ul of antibody in 40ul of sample)

Blocking Agent

1X Permeabilization buffer (BD Bioscience or ThermoFisher)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Alexa Fluor 700

Results Summary

This antibody is working well on mouse CD4 T cells.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

High quality

The Bad

Special buffers are required

The Bottom Line

Fixation/Permeabilization buffer set from either BD Bioscience or eBioscience (ThermoFisher) is needed for proper staining.

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