The Good
Only one antibody needed for detecting proliferation, rather simple, rather inexpensive, PE as a powerful conjugate
The Bad
Time-consuming fix/perm steps, excessive washing needed
The Bottom Line
For screening multiple conditions in an inexpensive way this antibody is really good. Staining is reproducible, antibody has high specificity, good value. Due to exzessive washing and long fix/perm steps this assay is not iseful for high throughput analysis.