CD31 Antibody Works For Flow Cytometry

Molecular Biology
Princeton
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Alexa Fluor® 647 anti-mouse CD31 Antibody

Catalog Number:

102416

This antibody was used on whole organ digests from mouse lung and bone marrow samples. It works and detects a subset of cells that are putative endothelial cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine lung and bone marrow digests

Primary Incubation

1:100

Blocking Agent

5% FBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

APC channel

Results Summary

Works by flow, although a dilution of 1:25-1:50 is recommended. Note that these cells will not survive FACS due to shear stress. Collagenase/trypsin digest does not hide the epitope.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works by flow, simple to use

The Bad

Does not stain cells strongly

The Bottom Line

Works, use a high concentration

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