CD62E (E-selectin) Antibody

September 20, 2017

Molecular Biology
Princeton
Graduate Student

Overall

Quality of Results

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Company:

Biolegend

Product Name:

CD62E (E-selectin) Antibody

Catalog Number:

148801

I tested this antibody against mouse bone marrow for immunofluorescence and TNF-stimulated HUVEC cells for western blotting. It does not work for any of the tested applications. The only working antibody is from BDPharmigen Clone 10E9.6 which works well for immunofluorescence.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse trabecular bone

Primary Incubation

1:50 in 20% goat serum

Blocking Agent

Goat serum

Secondary Incubation

Biotin anti-mouse

Tertiary Incubation

Streptavidin HRP

Detection

DAB reagent

Results Summary

No visible staining detected in samples that were known to be positive. Same result for western blot and immunofluorescence.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Nothing

The Bad

Doesn't work

The Bottom Line

Order clone 10E9.6 from BDPharmigen

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