BD Biosciences
CD25 antibody
555434
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Non-Human Primates peripheral blood was used to isolate mononuclear cell cultures (MNCs) using Ficoll gradient density centrifugation. The MNCs were activated using Con A for 3 -5 days and then the activation of cells was checked using CD25 antibodies.
Flow Cytometry
Peripheral Blood Mononuclear Cell Cultures from Non-Human Primates
5ul of antibody for 100ul of cells in FACS buffer for 15 mins at room temperature
None
MAQSQuant
The antibody was very good in separating the postive and negative populations.
N/A
Very easy to use and optimize
This is a very good antibody for checking activated T cells.