Invitrogen Antibodies by Thermo Fisher Scientific
Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor 488
A28175
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: IgG Immunofluorescence Antibodies
This secondary antibody was used to perform indirect immunofluorescence microscopy on HeLa cells to detect the ER/mitochondria. It showed strong immunoreactivity that was consistent mitochondria. Background levels were low. This antibody is a good choice for immunofluorescent microscopy.
Immunofluorescence
HeLa cells fixed with 3.7% paraformaldehyde
1 hour at room temperature in blocking agent at 1:300
PBS in 1% normal goat serum and 0.1% triton x-100
1 hour at room temperature (1:300)
DAPI
Confocal microscopy
Strong, specific and bright: Alexa 488 is a quality choice, but you pay a premium.
N/A
Good and reliable
Pricey
There are cheaper alternatives but this secondary antibody gives reproducible results