Great Human CD4 Antibody For Flow Cytometry

Pediatrics UH
Case Western Reserve University
Senior Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biosciences

Product Name:

Anti-Human CD4 RedFluor710

Catalog Number:

80-0048-T100

We use this antibody as a replacement for an antibody (same clone) from some of the bigger antibody companies (i.e. BD, eBioscience, Beckman Coulter etc ) in our human T cell multi-color (>10 color) flow cytometry staining panels. It works exactly the same as the more expensive antibody. I will continue to replace our antibodies with Tonbo equivalents as they are the same quality for a fraction of the price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human peripheral blood mononuclear cells

Primary Incubation

5ul per million cells for 15mins at Room Temp

Blocking Agent

2% FBS in PBS

Secondary Incubation

N/A is a conjugated antibody

Tertiary Incubation

N/A is a conjugated antibody

Detection

Flow cytometer - Attune NXT

Results Summary

Anti-Human CD4 RedFluor710 works perfectly in a T cell antibody panel. There is great separation between CD4 versus CD8 as shown in dot plot, as well as viewing by histogram.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great separation, low background

The Bad

Nothing that I can find

The Bottom Line

I will continue to purchase this antibody.

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