Monoclonal B-Actin Antibody

September 05, 2017

BlueskyReddit
Radiation Oncology
UAB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

Monoclonal anti-B-actin antibody produce in mouse

Catalog Number:

A5441

Browse Similar Products: beta Actin Western Blot Antibodies

An excellent antibody to detect B-actin, used for loading control/normalization in western blotting. It is a highly sensitive antibody.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human ovarian cancer cells

Primary Incubation

1:5000, 1 hour on shaker at room temperature

Blocking Agent

5% non-fat milk in TBS-T

Secondary Incubation

1:5000, 1 hour on shaker at room temperature

Tertiary Incubation

N/A

Detection

Pierce ECL Western Blotting Substrate

Results Summary

All four lanes represent ovarian cancer cells treated with different agents. B-actin is even across all four lanes, indicating equal loading.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Sensitive, highly specific antibody

The Bad

Must use small amount so challenging to avoid multiple freeze-thaw cycles

The Bottom Line

An excellent highly sensitive antibody to be used for detection of consistent loading in western blots

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