Zymo Research
DNA clean and concentrator-5
D4067
The goal was to clean up PCR products for downstream analysis. It worked perfectly. The concentration was enhanced better than anticipated.
DNA cleanup
PCR product
Add 1 part PCR product to 5 parts binding buffer and place into a spin column and spin for 30seconds and discard flowthrough. Add 200ul washbuffer and spin for 30 seconds and discard flowthrough (do this 2+ times). Add elution buffer and let sit at room temperature for 1 minute. spin into a clean centrifuge for 30 seconds
Tips make sure you let the solution buffer incubate for 1 minute
Yielded quality concentrated DNA. Exceeded my expectations
N/A
Quick and easy protocol, great yield
Not bad
Would recommend to anyone needing to purify PCR products