Specific Antibody For Recognizing Endogenous Bcat1 Protein

chemical biology
Indian institute of chemical technology
SRF

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Company:

Sigma

Product Name:

Monoclonal Anti-BCAT1 antibody produced in mouse

Catalog Number:

SAB4200561

Browse Similar Products: BCAT1 Western Blot Antibodies

In many cancers, to get a selective growth advantage, metabolism is the key.The recent finding revealed that these Bcat1 protein levels are high in many cancers. We are looking in to see how Bcat1 protein regulation in cancer cells is different than in normal cells. We are doing experiments by using promoter assays and by doing immunoblot analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Hela cell lysate

Primary Incubation

1:1000 dilution, at room temperature for 2hr

Blocking Agent

Skimmed milk

Secondary Incubation

1:5000 dilution, 1 hr at room temperature

Tertiary Incubation

Not required

Detection

ECL solution

Results Summary

We got very good results with out ambiguity because the antibody is working without any nonspecific. The blots we got are of highly publishable quality.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

No need to validate this antibody

The Bad

No negative remarks

The Bottom Line

Highly recommended

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