BD Biosciences
FITC Annexin V Apoptosis Detection Kit I
556547
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FITC Annexin V Apoptosis Detection Kit can be used to observe extent of apoptosis in cancer cells following treatment of agents/drugs.
Apoptosis Detection
Human cancer cells
Resuspend cells in Binding Buffer, stain cells with Annexin V-FITC, PI, and both in combination. Run cells by flow cytometry.
None to note
Percentage of cells identified as apoptosis is determined by gating for double positivity for Annexin V-FITC and PI.
N/A
Easy to use, not long procedure
Optimization may be needed to truly identify correct settings for apoptosis
The kit is a nice tool for apoptosis identification in cancer cells following treatment with drugs/agents.