Useful Antibody To Mark Human CD103 Cells

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience™ from Invitrogen Antibodies

Product Name:

CD103 (Integrin alpha E) Monoclonal Antibody

Catalog Number:

46-1037-41

In our lab we study many markers of T cells at different activation states. CD103 is an important marker for us to characterize different T cell compartments. We chose this antibody because it’s very bright and works well within our panel to reduce spill over

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human spleen cells

Primary Incubation

Cells were stained in 100uL FACS buffer with antibody at 1:50; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.

Blocking Agent

FACS buffer

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

Using this antibody we are able to successfully identify a small population of CD103+ cells isolated from a human spleen.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright

The Bad

None

The Bottom Line

This antibody works well to mark human CD103+ cells and be able to visualize them.

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