SPR Immobilization For Affinity Analysis

Medicine
University of Pittsburgh
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bio X Cell

Product Name:

Anti PDL-1 Antibody

Catalog Number:

BE0101

We are developing engineered antibody for PET imaging for cancer detection by targeting PD L-1. We always use a anti PDL-1 antibody as a control to compare affinity and kinetics of our engineering antibody. The goal was to compare the affinity of our antibody with the commercially available antibody. The reason for selecting this product is because it's cheap and comes with high concentration.

Experimental Design and Results Summary

Applications

ChIP

Sample

Transfected proteins

Primary Incubation

Immobilization of CM5 chip

Blocking Agent

Block unspecific binding via IgG

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Via SPR immobilization levels

Results Summary

We get response unit after anti PDL-1 immobilization and it tells how much antibody is binding to the Chip. It is used as a standard to compare the affinity of unknown affinity samples.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Low cost and highly concentrated antibody

The Bad

Nothing so far

The Bottom Line

Best antibody at low cost

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