Good IFNy Staining

August 17, 2017

BlueskyReddit
GSAS
Yale University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience by Invitrogen Antibodies

Product Name:

IFN gamma Monoclonal Antibody (XMG1.2), PE-Cyanine7

Catalog Number:

A18713

My main goal was to validate if the antibody was working so I did a very simple experiment of in vitro T cell stimulation. P14 splenocytes (mouse transgenic T cells) were activated in vitro with Gp33 peptide for 24hour in the presence of BFA and stained for intracellular cytokine production.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

30min on ice

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII

Results Summary

Good IFNy staining

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good clear IFNy staining

The Bad

None

The Bottom Line

Recommend for intracellular flow staining.

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