Follicular B Cells After Immunization

August 23, 2017

BlueskyReddit
Immunology
Northwestern University
Grad Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

CD23 APC-Cy7

Catalog Number:

101629

The goal of the project was to determine the effects of immunization on the follicular B cell population in the spleen.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1: 100, 4 degrees, 30 min

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

In combination with CD21, CD23 clearly shows the population of follicular B cells in the spleen.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, clearly marks positive cells

The Bad

None

The Bottom Line

Good antibody for follicular B cell staining.

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