CD3+ T Cell Staining After Immunization

August 15, 2017

Immunology
Northwestern University
Grad Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience™ from Invitrogen Antibodies

Product Name:

CD3e PE-Cy7

Catalog Number:

25-0031-82

The goal of the project was to determine T cell subsets in mice after immunization.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1: 100, 4 degrees, 30 min

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

Clearly stains CD3+ T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, distinctly marks positive cells

The Bad

None

The Bottom Line

Great antibody for CD3 staining by flow.

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